Stool sample storage conditions for the preservation of Giardia intestinalis DNA
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چکیده
منابع مشابه
Stool sample storage conditions for the preservation of Giardia intestinalis DNA.
Stool is chemically complex and the extraction of DNA from stool samples is extremely difficult. Haemoglobin breakdown products, such as bilirubin, bile acids and mineral ions, that are present in the stool samples, can inhibit DNA amplification and cause molecular assays to produce false-negative results. Therefore, stool storage conditions are highly important for the diagnosis of intestinal ...
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Giardia intestinalis is a widespread parasitic protozoa which has great significance as a public health threat. Molecular diagnostics of stool sample can be unreliable because of the presence of inhibitors of enzymatic reactions. The aim of this study was to determine the effectiveness of selected pre-treatment methods of fecal samples for further PCR-based diagnostics of G. intestinalis, and t...
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Assistant Surgeon, Medical College Hospital, Calcutta, ortnerly Clinical Pathologist, Protozoology Depart-' ment, Calcutta School of Tropical Medicine.) The; role which Giardia inrcsnnuus may 'n the production of intestinal symptoms is still a disputed point. Nothing very definite can be stated one way or the other. It is claimed on the one hand' that it is frequently present in healthy persons...
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Giardia intestinalis is a primitive microaerophilic protozoan parasite which colonises the upper intestine of humans. Despite the evolutionary and medical significance of this organism, its physiology is very poorly understood. In this study we have used a novel flow cytometric technique to make quantitative measurements of the electrical potential across the plasma membrane of G. intestinalis ...
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Background/aim: This study included patients who had digestive system complaints between August 2015 and October 2015. The research was designed to compare conventional microscopy with an antigen detection ELISA kit and the TaqMan-based real-time PCR (RT-PCR) technique for detection of Giardia intestinalis in human stool specimens. Materials and methods: Samples were concentrated by formalin-et...
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ژورنال
عنوان ژورنال: Memórias do Instituto Oswaldo Cruz
سال: 2012
ISSN: 0074-0276
DOI: 10.1590/s0074-02762012000800001